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In breast cancers, overexpression of HOTAIR increases metastatic potential via the repression of many additional genes. The regulated expression of the genome requires the formation of facultative heterochromatin fHC Trojer and Reinberg , which stably represses transcription. Although heterochromatin is essential for normal human development, dysregulation can promote cancer by altered expression of oncogenic and tumor suppressor genes. The recruitment of PRC2 to target genes results in the methylation of lysine 27 on histone 3 H3K27me , a histone mark that triggers formation of repressed chromatin Simon and Kingston PRC2 components are frequently up-regulated in multiple cancer types and can promote neoplastic transformation Varambally et al. However, PRC2 also readily binds to the nascent transcripts of actively transcribed genes Davidovich et al. More recently, this lncRNA has been shown to associate with hundreds of additional sites in the genome and to initiate broad changes in gene expression when overexpressed Gupta et al.
Aspects of the disclosure relate to computer systems, computer software, and video games. Video games are increasingly popular. Online multiplayer video games have become particularly popular due, at least in part, to the ability of players to compete with multiple other human players.
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